Bacteriophage genome packaging and ejection physics, role of osmosis

Author: Tsotne Dvali
Keywords: bacteriophages, biophysics, genome ejection, genome packaging
Annotation:

Structural-functional analysis of bacteriophages allows the process of energy conversion-storage in biosystems to be described at a fundamental level. The purpose of the paper is to analyze post-expression transformations of bacteriophages as a specific module of biosystems, in addition to the synthesis of existing experimental and therapeutic models. The main issue is the injection process, in relation to the energy stored in the packaging process, which is discussed in the revised 50-100 pn range in the paper. Most of the models used in the analysis are based on experimental simulated environments, as well as theoretical calculations from various simulations involving co-axial and toroidal organization.The results obtained from the simulated environmental experiments are compared with the models based on in vivo experiments and the results obtained. Most of the bacteriophages described are of a similar (bicuspid, spherical, oblong-type) type, which is explained by the complex diversity identified in the first stage of the analysis. The biosystem is described by the free energy change in both the two-part and three-part models. The gradient generated by osmotic and hydrostatic pressures is treated as an energy-induced process stored / converted in the packaging process. The injection and packaging forces of several bacteriophage genomes against environmental conditions are compared. One example is the epsiolon15 bacteriophage packing force ~ 110 pN, as a function of phage genome length, with a 64% contrast contribution to the osmotic pressure.