Quantitative indicators of SARS-Cov-2 virus amplicones in different groups of people

Author: mariam tchitanava
Keywords: SARS-CoV-2, COVID-19, RT-PCR, ELISA
Annotation:

Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Is the currently causing pandemic virus (2019-2022) that causes infectious disease COVID-19  (COronaVIrus Disease 2019). The severity of the disease caused by SARS-CoV-2 can be said to be unpredictable, as it has different effects - in people at different risk groups. It is known that COVID-19 pathogenesis is more severe in patients with various comorbidities than in humans without chronic disease. Due to the urgency of these events, we conducted a research in people with different risk groups. These risk groups are: diabetes, hepatitis C, hypercholesterolemia, lung cancer, autoimmune diseases. The control group consisted of people without concomitant disease. nosopharyngeal swabs of 5-5 patients were examined in each group on the 2nd day after the onset of symptoms. The aim of the study was to quantify SARS-CoV-2 virus amplifiers in people at different risk groups. Which was determined by the RT-PCR method (real- time polymerase chain reaction). The quantity of IgM antibodies o SARS-CoV-2 virus in the blood serum of the same people was then examined by ELISA (enzyme-linked immunosorbent assay). From the onset of symptoms, at different time intervals. Our aim was to determined a correlation between the results obtained from the studies conducted by these two methods. The data obtained showed that the number of SARS-CoV-2 virus amplifiers varied in people at different risk groups, and that serum IgM antibodies were elevated much later than nasopharyngeal smear PCR detection.

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