"Establishment of phylogenetic links between different fish species by modern molecular biology methods".

Author: nino nepharidze
Co-authors: -
Keywords: proteomics, hydrobionts, cladograms, western blot
Annotation:

The Human Genome Project has successfully completed the goal and aim of sequencing not several, but the all human genes. Far from closing the book, this body of work opened up a whole new field, which is modern molecular methods. Proteomics is the study of structure, function, and interaction of proteins with each other. Genes encode proteins, which serves function to determine an organism’s form, structure and function and phenotype–the raw material of natural selection. Changes in proteins can reflect changes in the gene pool. Also epigenetics could play a big role, which reflects on protein functions. Science has been trying to find relativeness through species, and creating systematics.. this classification also helps us identify evolutionary relativeness, which is components of creating life. Development of science could now exchange morphological analyses with modern molecular methods, such as sequencing and having knowledge about specific proteins and their polymorphism. the aim of the presented work was to study phylogenetic relationships among species, using modern methods of biology. The main task was to identify myosin from the samples selected from the Agrarian markets by the western blot method, process the results with the BLAST system and build a phylogenetic tree. We set the tasks to achieve goals:  Separation of proteins from species.  Electrophoresis from extracted proteins, then identifying myosin molecule using western blot method  Then Using Internet-based bioinformatics databases, we can now compare experimentally determined results to actual protein sequence data and consider whether variations in myosin between species are due to “genetic” or “epigenetic” factors