Study of the dynamics of antiphage antibody production in a mouse model against infections caused by Pseudomonas aeruginosa and Acinetobacter baumannii

Author: Sopiko Sturua
Co-authors: Sophiko Sturua
Annotation:

Pseudomonas aeruginosa and Acinetobacter baumannii play an important role in the etiology of nosocomial infections, therefore fighting against them is a priority of clinical medicine. Both microorganisms are resistant to too many antibiotics. The worldwide spread of these opportunistic pathogens and antibiotic-resistant bacterial strains has renewed interest in phage therapy. In order to introduce a new phage drug in the clinic, it is necessary to go through the previous experimental steps in terms of efficacy and safety of the drug. The object of this study was specific phages of Acinetobacter baumannii and Pseudomonas aeruginosa, called: BT-8 and Vb-AbS. For these phages, we have studied the different biological features: Obtaining a clean line of bacteriophages, Multiplication (Concentration), lysine spectrum and Lysine stability in the liquid nutrient agar, for which we have used semi-purified phagic drug “phagelysate”. We used pure-line female mice (CD1) in animal model experiments. We were using subcutaneous injections, both separately and in the form of a cocktail. One of the objectives of the research was to study the dynamics of the formation of antiphage antibodies in a mouse model with a single phage injection. Quantitative determination of the produced antibodies in the mouse serum on the 14th, 21th, 30th day by the method of phage neutralization. The results showed that the presence of neutralizing antibodies in the mouse's blood serum was quite high from the 14th day and reached a peak on the 21rd day. Based on the biological characteristics of BT-8 and Vb-AbS phages, and the fact that they have the ability to rapidly penetrate into the blood of experimental animals, we can conclude that these specific phages have the potential for therapeutic use. The presence of neutralizing antibodies in the blood serum of experimental animals shows an adequate immune response of the mouse organism to the phage nucleocapsid.