Functional changes in glutamate receptors in the hippocampus under conditions of chronic stress

Author: Temuri Mantashashvili
Co-authors: Nana Koshoridze, Giorgi burjanadze
Keywords: Psycho-emotional stress, Social isolation, glutamate receptor, NMDA receptor, Na/K-ATP-ase
Annotation:

Prolonged social isolation can have lethal consequences in both animal and human models, manifested in the changes of complex molecular mechanisms that take place in various tissues of the body, including the nervous tissue. This study showed that a prolonged 30-day social isolation leads to enhanced NMDA-receptor expertise of the hippocampal plasma membrane of the brain of experimental animals. It is known that the NMDA-receptor plays an important role in the regulation of synaptic plasticity and memory. Its activation significantly alters the intracellular Na+ and K+ concentrations, which are subsequently restored by the action of Na+/K+ -ATPase. We studied the degree of expression of NMDA-receptor subunits in rat hippocampal cells under conditions of 30-day social isolation. It has been observed that enhanced expression of the receptor NMDA-N1 and NMDA-2B subunits is observed under conditions of social isolation. However, similar changes were not observed in the NMDA-A2 subunit. In parallel, changes in Na+/K+-ATPase activity have also been studied. It has been observed that in contrast to the receptor, there is a decrease in enzyme activity. Using Wester-Blot analysis, it was observed that the reason for the change in enzyme activity was a decrease in the degree of expression of its α-subunit.

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