CD180 Toll-like receptor expression and function in monocytes of patients with active pulmonary tuberculosis

Author: Nino Sakidze
Keywords: CD180, Toll like receptors, tuberculosis
Annotation:

Georgia belongs to the high tuberculosis (TB) incidence countries with high proportion of multiple drug resistance cases. Hence studying immune responses to Mycobacterium tuberculosis (MTb) in Georgian population is of a paramount importance. TLRs (Toll like receptors) is important in the host's immune response against mycobacterial infection. Pathogen-associated molecules combine with TLRs and the Toll-interleukin 1 (IL-1) receptor (TIR) domains of myeloid differentiation protein 88 (MyD88). This interaction activates IL-1 receptor-related kinase (IRAK-1), tumor necrosis factor-α (TNF-α)-related factor (TRAF), and interferon-β (IFN-β)- induced TIR-carrying molecule (TIRAK) and conveys TLR activation toward the nucleus. Nuclear factor-κB, TNF-α, and IL-1 then initiate the immune response to M. tuberculosis in the nucleus. TLR-dependent NF-κB signaling and MAPK pathways contribute to antimycobacterial innate immunity through secretion of antibacterial effector molecules, cytokines, and chemokines, thus recruiting various immune cells to the site of infection. The aim of this research was to assess the frequency and phenotype of the monocytes and their role in the immunopathogenesis of the active form of the pulmonary TB. In this research was studied CD180 Toll-like receptor expression and function in monocytes of patients with active pulmonary tuberculosis, also assessed the frequency and phenotype of the monocytes and B lymphocytes, CD3+ T lymphocytes. As a result, In the peripheral blood of people with active pulmonary tuberculosis, there is a decrease in the frequency of monocytes compared to healthy controls; There is also a decrease in the expression of CD180 on monocytes and B cells. Probably, the frequency of lymphoid and myeloid cells decreases by apoptosis.

Lecture files:

CD180 თოლლ-მსგავსი რეცეპტორის ექსპრესია და ფუნქცია ფილტვის აქტიური ტუბერკულოზით დაავადებული პირების მონოციტებზე [ka]