Total and anti-Spike 1 & 2 IgG antibody titers against SARS CoV 2 in different research groups

The pandemic caused by the new coronavirus (SARS CoV 2) has put the world in front of the biggest challenge. Infection caused by coronavirus has become the most important problem for mankind. Therefore, the search for new different ways to prevent infection has become inevitable, timely and unavoidable. Vaccination is considered the most optimal way to prevent infection caused by SARS CoV 2 and the morbidity and mortality caused by it. Antibodies are produced by the body's immune system in response to the antigens of a pathogen (for example: virus, bacteria), as a result of infection or vaccination. At this stage, the following types of vaccines are available in Georgia: Pfizer-BioNTech, Sinopharm and Sinovac (CoronaVac -Vero Cell), Astrazeneca. Most of vaccines produce antibodies only Spike protein towards. The spike protein is the main molecule that is located on the surface of the SARS-CoV-2 virus, and it’s helps the virus with penetrate the host cells and spread throughout the body. The aim of our study was to determine the general and specific index of humoral immune memory against SARS CoV 2 in the blood samples of volunteers, and to achieve this goal we examined the peripheral blood samples of 170 volunteers for general and Spike 1&2 specific IgG of COVID-19. We divided the volunteers into the following groups: non-vaccinated covid-infected, vaccinated and co-infected persons, vaccinated only, simultaneously unvaccinated and non-infected persons. As a result of the research, it was established that: 1. All three types of SARS CoV 2 vaccines are available in Georgia: based on m-RNA, vector and inactivated virus effectively induce humoral immune memory, which lasts at least 6 months, and the circulating general and spike protein IgG levels are higher in vaccinated individuals. than only in infected persons; 2. In individuals with disorders of thyroid and/or pancreas functions, lower titers of both general and virus-specific proteins were observed, which makes it possible to assume that the dysfunction of these systems reduces the formation of humoral immune memory; 3. The spike protein of the SARS CoV 2 virus, against which the mRNA and vector vaccines were designed, is indeed a different antigen for this particular strain of these coronaviruses, against which the cross-reactive immune rate in the population is very low, and vaccines directed against the spike protein had a positive effect on the stimulation of the immune response.